WAV-1 UV/VIS Advanced Holmium Oxide Wavelength Accuracy Standard (240-640nm)


WAV-1 UV/VIS Advanced Holmium Oxide Wavelength Accuracy Standard (240-640nm)


The WAV-1 UV-VIS allows calibration of wavelength accuracy in the ultra-violet (UV) and visible (VIS) range. FireflySci offers the only holmium glass reference standard in the world that has the highly demanded wavelength peak of 241.5nm for verifications relating to DNA measurements and other applications.

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Features of WAV-1 UV/VIS:

We provide the following NIST-compliant peak values for the wavelength accuracy calibration of spectrophotometers:  (Values below are examples)

  • 241.5 nm

  • 279.3 nm

  • 287.6 nm

  • 333.8 nm

  • 360.8 nm

  • 385.8 nm

  • 418.5 nm

  • 453.4 nm

  • 459.9 nm

  • 536.4 nm

  • 637.5 nm

All Firefly calibration standards are NIST traceable and NIST compliant. Performance of all Firefly reference calibration standards, including but not limited to this standard, meet or exceed NIST requirements.

Unless recalibrations on a periodic basis are required by QA procedures of a customer’s organization, all Firefly solid-state calibration standards do not need to be re-calibrated due to materiaI aging.

Where required, Firefly will provide periodic re-calibrations. Contact us for more information.

Reminder: The measured wavelengths’ peaks depend, among other factors, on the user’s spectrophotometer’s slit-width (spectral band-pass). Our calibration measurements are provided with at a 1 nm slit-width and could also be performed at the customer-desired slit-width(s) for an extra fee to be set on per-case basis

All calibration standards come with the following:

  • FireflySci Unique Lifetime Warranty

  • Certificate of Calibration

  • Instructions on how to properly use calibration standards

All of our calibration standards are proudly made in North America.

Instructional video on how to use our WAV-1 UV/VIS holmium oxide filter

Sample Absorbance and Wavelength Scans of the WAV-1 UV/VIS:

Absorbance Data

Absorbance Data

Transmission Data

Transmission Data


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