How to Calibrate a Spectronic 20D Spectrophotometer

Hi all you FireflySci Fans!

Here is our latest video and we demo how to do a calibration on an old Spectronic 20D spectrophotometer. This is a unique machine because it can use both cuvettes and test tubes. The test tubes are really long and if you plan on using a standard 10x10x45 mm cuvette in this machine you need to invest in the cuvette adapter. If you don’t, well let’s just say you’re going to have a lot fun trying to fish out the cuvette from the bottom of this cell holder.

Other than the interesting cuvette holder, the calibration is pretty straightforward. Check out the video below and of course please feel free to send us any questions. Our e-mail is